Take a sample from the mane. Directly before sampling, the mane should not be washed and only precisely combed to help remove dirt, dead hair, and other detritus.

Separate the first strand and cut it with sharp scissors just by the skin. For the test, send 1 inch of the strand, measuring from the skin side. The remaining part of the hair can be removed. Repeat this operation as many times as necessary, so that the weight of the sample is 300-400 mg, which corresponds to the volume of 1 tablespoon.

For a precise determination of the trends, repeat the test in 6 months.